FIGURE

Fig. 3.

ID

ZDB-FIG-200703-28

Publication

Sam et al., 2020 - Specificity, redundancy and dosage thresholds among gata4/5/6 genes during zebrafish cardiogenesis

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Fig. 3.

Gata5 and gata6 are redundant for cardiac specification. ISH was performed using offspring from a gata5^+/−, gata6^+/− in-cross with probes targeting either myocardial marker myl7 (left panels) or cardiac progenitor marker nkx2.5 (right panels). (A) gata5^−/−, gata6^−/− mutants exhibit a heartless phenotype and a complete loss of cardiac progenitors (indicated by arrows). Representative scale bar in WT panels: 0.1 mm. (B) Quantification of nkx2.5 staining shown in A. Error bars represent s.e.m. *P<0.05, ****P<0.0001. (C) Schematic showing that loss of gata5 and 6 disrupts the initial stages of heart development.

Expression Data

Genes:	myl7 nkx2.5
Fish:	WT gata5^wcm8/wcm8 gata6^wcm7/wcm7 gata5^wcm8/wcm8; gata6^wcm7/+ gata5^wcm8/wcm8; gata6^wcm7/wcm7
Anatomical Terms:	cardiac muscle cell heart primordium
Stage Range:	14-19 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gata5^wcm8/wcm8 gata5^wcm8/wcm8; gata6^wcm7/+ gata5^wcm8/wcm8; gata6^wcm7/wcm7
Observed In:	cardiac muscle cell cardiac muscle progenitor cell migration to the midline involved in heart field formation heart primordium heart tube myocardial precursor
Stage Range:	14-19 somites to Prim-15

Phenotype Detail

Acknowledgments

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