FIGURE

Figure 3

ID
ZDB-FIG-200318-12
Publication
Weinberger et al., 2020 - Functional Heterogeneity within the Developing Zebrafish Epicardium
Other Figures
All Figure Page
Back to All Figure Page
Figure 3

Transcriptionally Distinct Epicardial Subpopulations Epi1–3 Localize to Different Regions of the Developing Heart

(A) Marker gene expression in Epi1–3. Cells were clustered in an unsupervised manner (columns). Color key indicates range of log2 transformed FPKM values. Genes analyzed further are highlighted in red. CM, cardiomyocyte; eHC, erythroid hematopoietic cell; mHC, myeloid hematopoietic cell; NC, neural cell; and MC, mesenchymal cell.

(B) mRNA staining of the Epi1 markers adma (magenta), jam2b (red), and tcf21 (cyan) in a 5 dpf heart. (B′ and B″) A nucleus (asterisk) in the epicardial region surrounded by adma, jam2b, and tcf21.

(C) mRNA staining of the Epi2 marker elnb (magenta) and tbx18 (green) at 5 dpf. (C′ and C″) Overlap of elnb and tbx18 in the BA.

(D) Antibody staining of Mylka and tbx18:myr-Citrine at 5 dpf. (D′ and D″) Overlap of Mylka and myr-Citrine in the BA.

(E) mRNA staining of the Epi3 marker cldn11a (magenta) and tcf21 (cyan) at 5 dpf. (E′ and E″) Two nuclei (asterisks) in the epicardial region between BA and atrium in close proximity to cldn11a and tcf21. Scale bars: 20 μm in (B)–(E) and 5 μm in (B′)–(E′) and (B″)–(E″). Color channels were adjusted separately for brightness and contrast. (B)–(E) are single optical sections. V, ventricle; BA, bulbus arteriosus.
Expression Data
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 52(5), Weinberger, M., Simões, F.C., Patient, R., Sauka-Spengler, T., Riley, P.R., Functional Heterogeneity within the Developing Zebrafish Epicardium, 574-590.e6, Copyright (2020) with permission from Elsevier. Full text @ Dev. Cell
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.