FIGURE

Figure 1

ID

ZDB-FIG-200318-10

Publication

Weinberger et al., 2020 - Functional Heterogeneity within the Developing Zebrafish Epicardium

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Figure 1

Heterogeneous Expression of tcf21, tbx18, and wt1b in the Developing Zebrafish Epicardium

(A) Fluorescence of tcf21:myr-tdTomato (magenta membrane), tbx18:myr-eGFP (green membrane), and wt1b:H2B-Dendra2 (green nucleus).

(B) Workflow to establish TgBAC(tcf21:myr-tdTomato;tbx18:myr-eGFP;wt1b:H2B-Dendra2)^ox187. (C) Schematic of epicardial fluorescence patterns and cardiac regions analyzed. dsBA, distal bulbus arteriosus; prBA, proximal bulbus arteriosus; apV, arterial pole of the ventricle; and AVC, atrioventricular canal.

(D–F) Single optical sections of mRNA stainings of (D) tcf21 (cyan) and tcf21:myr-tdTomato (magenta), (E) tbx18 (magenta) and tbx18:myr-eGFP (green), and (F) wt1b (magenta) and wt1b:H2B-Dendra2 (green). (D′–F′) Cell nuclei (asterisks) in the epicardial region surrounded by endogenous mRNA and fluorophore mRNA/protein.

(G–I) Projections of the heart in triple reporter larvae at 3 dpf (G), 5 dpf (H), and 7 dpf (I).

(G′ and H′) Single optical sections from (G) and (H).

(I′) Short projection from (I).

(G″–I″ and G‴–I‴) Representative epicardial fluorescence patterns.

(J–L) Relative quantification of epicardial fluorescence patterns across the regions indicated in (C) at 3 dpf (J), 5 dpf (K), and 7 dpf (L).

Scale bars: 20 μm in (D)–(F), 5 μm in (D′)–(F′), 50 μm in (G)–(I) and (G′)–(I′), and 10 μm in (G″)–(I″) and (G‴)–(I‴). Color channels were adjusted separately for brightness and contrast. Data in (J)–(L) are represented as mean minus standard deviation. Number of embryos analyzed: 3 dpf n = 6, 5 dpf n = 10, and 7 dpf n = 6. V, ventricle; BA, bulbus arteriosus.

See also Figure S1.

Expression Data

Genes:	Dendra2 EGFP tcf21 Tomato
Fish:	ox181Tg ox181Tg; ox184Tg; ox186Tg
Anatomical Terms:	epicardium epicardium nucleus epicardium plasma membrane
Stage Range:	Protruding-mouth to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 52(5), Weinberger, M., Simões, F.C., Patient, R., Sauka-Spengler, T., Riley, P.R., Functional Heterogeneity within the Developing Zebrafish Epicardium, 574-590.e6, Copyright (2020) with permission from Elsevier. Full text @ Dev. Cell