The expression profiles of zebrafish lrrc8aa and lrrc8ab. (A) The expression levels of lrrc8 family during zebrafish embryogenesis in transcripts per million (TPM) were obtained from an Expression Atlas. Lrrc8aa is much more abundant than lrrc8ab in the first 24 h of development. While lrrc8c is the most abundant family member during gastrulation; the expression levels are comparable after 24 hpf (prim-5). (B) The sequencing result did not detect lrrc8ab in zygotes and at the two-cell stage, but RT-PCR with β-actin (actb1) as the loading control showed that both lrrc8a genes are maternal messages and can be detected at all developmental stages through to 72 hpf. (C–L) Whole-mount in situ hybridization of lrrc8aa (C,E,G,H,J,K) and lrrc8ab (D,F,I,L) showed that both genes are ubiquitously presented in the zebrafish embryo at 6 (C,D) and 12 (E,F) hpf. In 24 hpf embryos (G–I), both genes could be clearly detected at the ventricular layer of the brain as well as the cardiogenic region. In 48 hpf embryos (J–L), both genes could be detected at brain ventricles, retina, otic vesicles and pectoral fin buds. Scale bars: 200 µm.
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