FIGURE

Figure 2.

ID

ZDB-FIG-200205-25

Publication

Nichols et al., 2020 - Functional Regeneration of the Sensory Root via Axonal Invasion

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Figure 2.

Taxol Induces Actin-Rich Invasion Components during Spinal Re-entry.

(A–D) Z-projection time-lapse images of avulsed DRG in Tg(sox10:gal4); Tg(uas:lifeact-gfp) animals treated with DMSO (A), Taxol (B), Taxol+GM6001 (C), and Taxol+SU6656 (D). Graphs show Lifeact-GFP intensity tracings during regeneration. White boxes denote insets of the growing axon and growth cone regions. The green arrow denotes a stable invasive structure. Red arrows and brackets represent peaks and durations of peaks of Lifeact-GFP.

(E) Graph of spinal re-entry in axons treated with DMSO, Taxol, Taxol+GM6001, and Taxol+SU6656.

(F) Duration of actin concentrates in DMSO axons (n = 7), Taxol axons (n = 6), Taxol+GM6001 axons (n = 6), and Taxol+SU6656 axons (n = 4).

(G) Representative scatterplot of growth cone area and average Lifeact-GFP intensity during regeneration in an axon treated with Taxol. Each time point is represented by a point.

(H) Percentage of time points for which the regenerating growth cone displayed each actin organization. Tukey’s HSD was used in (D) and two-way ANOVA was used in (H). Scale bar, 10 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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