FIGURE

Fig. 5

ID

ZDB-FIG-191230-671

Publication

Pérez-Rius et al., 2019 - Comparison of zebrafish and mice knockouts for Megalencephalic Leukoencephalopathy proteins indicates that GlialCAM/MLC1 forms a functional unit

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Fig. 5

Mlc1 is mislocalized in primary Glialcam^−/− astrocytes. Localization of GlialCAM (a) and Mlc1 (b) in primary astrocytes from wild-type (WT, left), Glialcam^−/− (middle) and Glialcam^−/− complemented with adenoviruses expressing human GlialCAM (right). In WT and complemented astrocytes, GlialCAM and Mlc1 are located at cell-cell junctions (arrowheads). Scale bar: 10 μm. (c) GlialCAM and Mlc1 protein levels primary astrocytes from wild-type (WT, left), Glialcam^−/− (middle) and Glialcam^−/− complemented with an adenoviruss expressing human GlialCAM. Actin served as a loading control. Two other independent experiments gave similar results. Densitometric analysis (n = 3) indicates that Mlc1 levels were reduced in astrocytes from Glialcam^−/− mice and expression was recovered after expression of GlialCAM using adenoviruses. * p < 0.05 vs wild-type astrocytes

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Orphanet J Rare Dis