Fig. 5

Fig. 5

Mlc1 is mislocalized in primary Glialcam^−/− astrocytes. Localization of GlialCAM (a) and Mlc1 (b) in primary astrocytes from wild-type (WT, left), Glialcam^−/− (middle) and Glialcam^−/− complemented with adenoviruses expressing human GlialCAM (right). In WT and complemented astrocytes, GlialCAM and Mlc1 are located at cell-cell junctions (arrowheads). Scale bar: 10 μm. (c) GlialCAM and Mlc1 protein levels primary astrocytes from wild-type (WT, left), Glialcam^−/− (middle) and Glialcam^−/− complemented with an adenoviruss expressing human GlialCAM. Actin served as a loading control. Two other independent experiments gave similar results. Densitometric analysis (n = 3) indicates that Mlc1 levels were reduced in astrocytes from Glialcam^−/− mice and expression was recovered after expression of GlialCAM using adenoviruses. * p < 0.05 vs wild-type astrocytes