Senescence detection in rag1−/− zebrafish and reversion by senolytic and antioxidant treatments. (a–c) Expression of the senescence marker genes tp53, mdm2, and CIP/KIP (p21, p27, p57) and INK4 (cdkn2a/b, cdkn2c) inhibitors in the visceral organs of wt and rag1−/− zebrafish. (a) Comparison of the expression in wt and rag1−/− fish under naïve conditions. (b) Effect of the one‐month‐long treatment with ABT‐263 and ALCAR in wt and (c) rag1−/− zebrafish. The expression level of each gene was normalized to 18s gene expression and is expressed as the fold change with respect to the levels detected in the corresponding control group (a: wt; b: wt control; c: rag1−/− control). (d, e) SA‐β‐gal activity in the skin of one‐year‐old zebrafish. (d) Differences in the percentage of β‐gal‐positive pixels between wt and rag1−/− fish under naïve conditions. (e) Effect of the one‐month‐long treatment with ABT‐263 and ALCAR in wt and rag1−/− zebrafish. The graphs represent the means ± SEM of five independent biological replicates. Significant differences are displayed as **(0.001 < p < 0.01) or *(0.01 < p < 0.05)
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