FIGURE

Figure 5

ID

ZDB-FIG-191230-1379

Publication

Chen et al., 2019 - Loss of rps9 in Zebrafish Leads to p53-Dependent Anemia

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Figure 5

Anemic phenotype is partially rescued by p53 konckdown in rps9 deficient embryos. (A-C) Whole mount in situ shows that p53, p21, and mdm2 are upregulated in mutants. (D) The expression of p53 and p53 target genes including mdm2 and p21 increases as measured by qPCR. The relative fold induction is represented as fold change over sibling control. (E) p53 inhibition by MO injection knockdown the gene expression of p53 and p53-target genes. (F) The morphological defects of rps9 mutant are largely rescued by p53 inhibition. (G-H) An increase of hemoglobin level appears at 48 hpf. (G) and 3 dpf (H) after p53 knockdown.

Expression Data

Genes:	cdkn1a mdm2 tp53
Fish:	AB rps9^la490/la490 (AB) rps9^la491/la491 (AB) rps9^la490/la490 + MO4-tp53 (AB) rps9^la491/la491 + MO4-tp53 (AB)
Knockdown Reagent:	MO4-tp53
Anatomical Terms:	head whole organism
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rps9^la490/la490 (AB) rps9^la491/la491 (AB) rps9^la490/la490 + MO4-tp53 (AB) rps9^la491/la491 + MO4-tp53 (AB)
Knockdown Reagent:	MO4-tp53
Observed In:	head pericardium trunk whole organism whole organism hemoglobin
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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