Fig. 2

bubblebrain Functions Downstream of Cell Death and Engulfment

(A and B) Dorsal view of blb^NY007; Tg(spi1b:Gal4,UAS:GFP); Tg(NBT:DsRed) (A) and blbNY007; Tg(spi1b:Gal4,UAS:GFP); Tg(NBT:DsRed) injected with a Caspase 3 morpholino and treated with Z-VAD to reduce apoptosis (B); representative images quantified in (G).

(C and D) Microglial cells labeled with Tg(spi1:Gal4,UAS:GFP) in 4 dpf blb^NY007 (C) and in blb^NY007 injected with morpholinos against BAI1 and TIM-4 to reduce engulfment (D); representative images quantified in (H).

(E and F) Representative examples of trunk 4 dpf macrophages in blb^NY007 (E) and blb^NY007 treated with Camptothecin to increase apoptosis (F). Scale bar 10 μm.

(G) Quantification of (A) and (B). Number of microglia with bubble morphology in blb^NY007 (n = 61) and blb^NY007 with reduced apoptosis (n = 61). Whiskers: 5th/95th percentile, p value < 0.0001.

(H) Quantification of (C) and (D). Number of microglia with bubble morphology in blb^NY007 (n = 50) and blb^NY007 injected with Tim4+BAI1 morpholino to reduce engulfment (n = 50). Whiskers: 5th/95th percentile, p value < 0.0001.

(I) Electron micrograph of a representative blb^NY007 microglia at 3 dpf. Scale bar, 5 μm.