ZFIN Image: Villani et al., 2019, Fig. 2

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Figure Caption

Fig. 2 bubblebrain Functions Downstream of Cell Death and Engulfment

(A and B) Dorsal view of blb^NY007; Tg(spi1b:Gal4,UAS:GFP); Tg(NBT:DsRed) (A) and blbNY007; Tg(spi1b:Gal4,UAS:GFP); Tg(NBT:DsRed) injected with a Caspase 3 morpholino and treated with Z-VAD to reduce apoptosis (B); representative images quantified in (G).

(C and D) Microglial cells labeled with Tg(spi1:Gal4,UAS:GFP) in 4 dpf blb^NY007 (C) and in blb^NY007 injected with morpholinos against BAI1 and TIM-4 to reduce engulfment (D); representative images quantified in (H).

(E and F) Representative examples of trunk 4 dpf macrophages in blb^NY007 (E) and blb^NY007 treated with Camptothecin to increase apoptosis (F). Scale bar 10 μm.

(G) Quantification of (A) and (B). Number of microglia with bubble morphology in blb^NY007 (n = 61) and blb^NY007 with reduced apoptosis (n = 61). Whiskers: 5th/95th percentile, p value < 0.0001.

(H) Quantification of (C) and (D). Number of microglia with bubble morphology in blb^NY007 (n = 50) and blb^NY007 injected with Tim4+BAI1 morpholino to reduce engulfment (n = 50). Whiskers: 5th/95th percentile, p value < 0.0001.

(I) Electron micrograph of a representative blb^NY007 microglia at 3 dpf. Scale bar, 5 μm.

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Acknowledgments

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Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell