FIGURE

Fig. 3

ID

ZDB-FIG-191224-2

Publication

Villani et al., 2019 - Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment

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Fig. 3

Cloning and Characterization of Slc37a2, the Gene Underlying the blb Phenotype

(A) Schematic of the slc37a2 locus. Sequences of the NY007/t³⁰³⁰¹ allele (C/T mutation) and NA150/t³⁰⁹¹³ allele (T/A mutation).

(B) Dorsal view of a 4 dpf Slc37a2 knockout generated with CRISPR-Cas9 as shown in (A). Scale bar, 30 μm.

(C) Dorsal view of a representative immunostaining of a 4 dpf embryo where microglia are in green Tg(mpeg:GFP-caax) and Slc37a2 in red. Scale bar, 30 μm.

(D) Magnification of (C). Scale bar, 15 μm. The yellow arrow points to a phagosome positive for Slc37a2.

(E) Western blot analysis of Slc37a2 (55 kDa) and Tubulin (48 kDa) in wild-type, blb^NY007, and blb^NI150.

(F) Immunostainings of Slc37a2 (cyan) and Lamp1 (red) in RAW264.7. Scale bar, 5 μm.

(G and H) Mass-spectrometry data showing normalized intensities of glucose 6-phosphate (G) not significant, and D-gluconate-6-phosphate (H) significant, p value < 0.0001, in blbNY007 and wild-type.

Expression Data

Genes:	EGFP slc37a2
Antibody:	Ab2-slc37a2
Fish:	WT slc37a2^{t30301/t30301} slc37a2^{t30913/t30913} zh901Tg
Anatomical Terms:	microglial cell microglial cell phagocytic vesicle whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc37a2^{t30301/t30301} slc37a2^{t30913/t30913}
Observed In:	head 6-phospho-D-gluconate whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 49(1), Villani, A., Benjaminsen, J., Moritz, C., Henke, K., Hartmann, J., Norlin, N., Richter, K., Schieber, N.L., Franke, T., Schwab, Y., Peri, F., Clearance by Microglia Depends on Packaging of Phagosomes into a Unique Cellular Compartment, 77-88.e7, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell