FIGURE

Fig. 4

ID

ZDB-FIG-191021-14

Publication

Lee et al., 2019 - A thrombomodulin-like gene is crucial to the collective migration of epibolic blastomeres during germ layer formation and organogenesis in zebrafish

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Fig. 4

The impact of zTM-b knockdown to vessels formation. Tg ( fli 1:eGFP) embryos were injected with TM-b MO with/without co-injecting plasmids encoding human TM, as described in Material and Methods, and observed for the development of ISV at 28 hpf ( a- c) and CCV at 56 hpf ( d, e). The percentage of morphants displaying anomaly in ISV (arrowheads) and CCV (CCV boundary circled by solid line) were also recorded. The extent of incomplete extension observed in ISV was quantified by calculating the length (yellow double-headed arrows) of five ISV singlets (brackets) immediately adjacent to cloaca for each morphant. f, g Larvae at 3 dpf were video-recorded laterally for the trunk area with anterior to the right for blood flow. A single red blood cell (boxed in red) inside the caudal vein was traced and calculated for its traveling distance (F; double-headed arrows) and velocity ( g) by analyzing the serial video still frames within 0.167 s. Presented are the results collected from at least three independent experiments including total 16–35 larvae for each experimental group. WT, wild-type embryos; MO, zTM-b morphants; hTM, human thrombomodulin

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cd248b y1Tg + MO1-cd248b
Knockdown Reagent:	MO1-cd248b
Observed In:	blood circulation common cardinal vein intersegmental vessel
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.