Fig. S1

Effects of the Mtz treatment, Related to Figure 1. (A) Mtz treatment was ineffective to the trunk vasculature. (B and C) Compared to DMSO treatment (B, n=20/20), the body phenotypes of larvae treated with Mtz were normal from 1 dpt to 5 dpt (C, n=20/20). Lateral view, anterior left. (D) Boxes on the brain vasculature map indicate image areas in corresponding panels. (E and F) In contrast to DMSO treatment (E, n=32/32), widespread TUNEL signals were detected in the injured brain area including endothelial and neuronal tissues at 1 dpt after Mtz treatment, but not in the trunk vasculature (F, n=29/30). Scale bar, 50 μm. Ventral view, anterior upward. (G and H) In contrast to DMSO treatment (G, n=71/71), Mtz treatment led to hemorrhages (arrowhead) in the injured area (H, n=41/62) at 1 dpt. Dorsal view, anterior upward. (I and J) In contrast to DMSO treatment (I, n=23/23), macrophage infiltration and engulfment of dead cells (arrowheads) were observed after Mtz treatment (J, n=32/32). Dorsal view, anterior upward. (K) Perfusions of nascent blood vessels were initially detectable at 4 dpt under the Tg(gata1:DsRed; kdrl:DenNTR) transgenic background (arrows, n=10/10). Dorsal view, anterior upward. Scale bar, 50 μm. AMCtA, anterior (rostral) mesencephalic central artery; BA, basilar artery; BCA, basal communicating artery; CtA, central artery; CVP, choroidal vascular plexus; MMCtA, middle mesencephalic central artery; MtA, metencephalic artery; PCS, posterior (caudal) communicating segment; PHBC, primordial hindbrain channel; PMCtA, posterior (caudal) mesencephalic central artery.