PUBLICATION

Cerebrovascular Injuries Induce Lymphatic Invasion into Brain Parenchyma to Guide Vascular Regeneration in Zebrafish

Authors
Chen, J., He, J., Ni, R., Yang, Q., Zhang, Y., Luo, L.
ID
ZDB-PUB-190423-1
Date
2019
Source
Developmental Cell   49(5): 697-710.e5 (Journal)
Registered Authors
He, Jianbo, Luo, Lingfei
Keywords
brain vascular regeneration, meningeal lymphatics, zebrafish
MeSH Terms
  • Animals
  • Apoptosis
  • Brain Injuries/etiology
  • Brain Injuries/therapy*
  • Cerebrovascular Trauma/complications*
  • Edema/etiology
  • Edema/therapy*
  • Lymphangiogenesis*
  • Lymphatic System/physiopathology*
  • Meninges/physiopathology*
  • Regeneration*
  • Zebrafish
PubMed
31006646 Full text @ Dev. Cell
Abstract
Damage to regional cerebrovascular networks and neuronal tissues occurs during acute cerebrovascular diseases, such as ischemic stroke. The promotion of vascular regeneration is the most promising therapeutic approach. To understand the cellular and molecular mechanisms underlying brain vascular regeneration, we developed two zebrafish cerebrovascular injury models using genetic ablation and photochemical thrombosis. Although brain parenchyma is physiologically devoid of lymphatic vasculature, we found that cerebrovascular injuries induce rapid ingrowth of meningeal lymphatics into the injured parenchyma. The ingrown lymphatics on one hand become lumenized to drain interstitial fluid to resolve brain edema and on the other hand act as "growing tracks" for nascent blood vessels. The ingrown lymphatic vessels undergo apoptosis and clearance after cerebrovascular regeneration. This study reveals a pathological function of meningeal lymphatics, through previously unexpected ingrowth into brain parenchyma and a newly identified lymphatic function as vascular "growing tracks."
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