Fig. 2 S2

( A) Blood vasculature (EC nuclei labelled in green) of sibling and MZ yap1mw48-/- mutant at 24 hpf. S1-4 indicate the quantified sprouts of the sibling and M1-4 the equivalent for the MZ yap1mw48-/- mutant in D–F). Dorsal aorta (DA). Scale bars in A–C): 50 μm. ( B) Blood vasculature of [ Tg(fli1a:nEGFP)] sibling and MZ yap1mw48-/- mutant at 32 hpf. Posterior cardinal vein (PCV). ( C) Trunk vasculature of sibling and MZ yap1mw48-/- mutant at 50 hpf showing normal lumen formation in white ([ Tg(kdrl:GFP)]). Missing parachordal LECs (PLs) are marked by asterisks. ( D) Quantification of number of nuclei for all sprouts (S1-S4 for sibling, M1-4 for MZ yap1mw48-/-) at 24 hpf (sibling: 2.73 ± 0.12, n = 56; MZ yap1mw48-/-: 2.14 ± 0.13, n = 56; p=0.0013 (**)) and 32 hpf (sibling: 2.93 ± 0.09, n = 44; MZ yap1mw48-/-: 2.91 ± 0.15, n = 44; p=0.90 (ns)). ( E) Sprout length for all sprouts in μm (S1-S4 for sibling, M1-4 for MZ yap1mw48-/-) at 24 hpf (sibling: 50.91 ± 1.70, n = 56; MZ yap1mw48-/-: 44.74 ± 2.32, n = 56; p=0.034 (*)) and 32 hpf (sibling: 85.57 ± 2.98, n = 44; MZ yap1mw48-/-: 74.57 ± 3, n = 44; p=0.01 (*)). ( F) Quantification of nuclei number in single sprouts at 24 hpf (S1: 2.64 ± 0.20, n = 14, M1: 2.64 ± 0.23, n = 14, p>0.99 (ns); S2: 2.64 ± 0.19, n = 14, M2: 2.07 ± 0.25, n = 14, p=0.08 (ns); S3: 2.93 ± 0.37, n = 14, M3: 2.00 ± 0.28, n = 14, p=0.05 (ns); S4: 2.71 ± 0.16, n = 14, M4: 1.86 ± 0.27, n = 14, p=0.01 (*)) and 32 hpf (S1: 3.27 ± 0.14, n = 11, M1: 2.64 ± 0.24, n = 11, p=0.04 (*); S2: 3.09 ± 0.16, n = 11, M2: 2.91 ± 0.31, n = 11, p=0.61 (ns); S3: 2.63 ± 0.15, n = 11, M3: 3.18 ± 0.23, n = 11, p=0.06 (ns); S4: 2.73 ± 0.20, n = 11, M4: 2.91 ± 0.39, n = 11, p=0.68 (ns)).