FIGURE

Fig. 4

ID

ZDB-FIG-190723-1403

Publication

Pei et al., 2019 - AP endonuclease 1 (Apex1) influences brain development linking oxidative stress and DNA repair

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Fig. 4

Fluorescent immunohistochemical staining of zebrafish brain for Apex1, Polb, and Creb1.

Standard fluorescent immunohistochemical staining was performed, and recorded by confocal microscopy (×40 magnification). Zebrafish embryos at the 1-cell stage were injected with control MO, 0.1, or 0.2 mM Apex1 MO, and allowed to develop for an additional 48 h before they were fixed and processed as described in Methods. Green fluorescence represents the expression pattern for glial fibrillary acidic protein (GFAP), while red fluorescence indicates the expression pattern for the indicated protein: Apex1 (a), Polb (b), or Creb1 (c), respectively (n = 20 in each group). d Western blot quantification of Apex1, Polb, or Creb1 (n = 30). Each experiment was repeated three times. Significant difference is indicated by *p < 0.05 and **p < 0.01

Expression Data

Genes:	apex1 cdkn1a creb1a egr2a mdm2 ogg1 polb tp53
Fish:	WT + MO4-apex1 WT + MO4-apex1 + MO4-tp53 WT + MO4-tp53
Knockdown Reagents:	MO4-apex1 MO4-tp53
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-apex1 WT + MO4-apex1 + MO4-tp53 WT + MO4-tp53
Knockdown Reagents:	MO4-apex1 MO4-tp53
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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