FIGURE

Fig. 5

ID

ZDB-FIG-190328-25

Publication

Zheng et al., 2018 - Syntaxin 1B Mediates Berberine's Roles in Epilepsy-Like Behavior in a Pentylenetetrazole-Induced Seizure Zebrafish Model

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Fig. 5

Berberine (BBR) inhibited seizure in PTZ-model zebrafish with the increase of STX1B level. (A) A behavioral experiment showed that BBR inhibited the larval overexcited locomotion in speed and distance under the conditions of non-stimulation and dark–light cycling stimulation in PTZ-model larvae. A representative swimming trajectory (2 min) is presented (n = 24). The rectangles showed differential distances and speeds between light–dark transitions in the groups of PTZ plus BBR larvae as compared with in the PTZ-only model. (B) Hybridization in situ showed that BBR inhibited the increase of c-fos and rescued stx1b descending induced by PTZ in the larval (7 dpf) brains (n = 20). (C) Western blotting results confirmed that BBR recovered STX1B protein levels to almost normal in a dose-dependent manner (n = 3). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 vs. wild-type; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. PTZ model; ^§ P < 0.05, ^§§ P < 0.01, and ^§§§ P < 0.001 vs. wild-type in the light condition; ^ΦP < 0.01 and ^ΦΦP < 0.01 indicates light vs. dark.

Expression Data

Genes:	fosab stx1b
Antibody:	Ab1-stx1b
Fish:	AB
Conditions:	chemical treatment by environment: pentetrazol chemical treatment by environment: berberine
Anatomical Terms:	brain whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment by environment: pentetrazol chemical treatment by environment: berberine
Observed In:	brain locomotory behavior whole organism
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.