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Fig. 5
Berberine (BBR) inhibited seizure in PTZ-model zebrafish with the increase of STX1B level. (A) A behavioral experiment showed that BBR inhibited the larval overexcited locomotion in speed and distance under the conditions of non-stimulation and dark–light cycling stimulation in PTZ-model larvae. A representative swimming trajectory (2 min) is presented (n = 24). The rectangles showed differential distances and speeds between light–dark transitions in the groups of PTZ plus BBR larvae as compared with in the PTZ-only model. (B) Hybridization in situ showed that BBR inhibited the increase of c-fos and rescued stx1b descending induced by PTZ in the larval (7 dpf) brains (n = 20). (C) Western blotting results confirmed that BBR recovered STX1B protein levels to almost normal in a dose-dependent manner (n = 3). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 vs. wild-type; #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. PTZ model; § P < 0.05, §§ P < 0.01, and §§§ P < 0.001 vs. wild-type in the light condition; ΦP < 0.01 and ΦΦP < 0.01 indicates light vs. dark.