FIGURE

Fig. 8

ID

ZDB-FIG-181101-17

Publication

Kaufman et al., 2018 - rbpms2 functions in Balbiani body architecture and ovary fate

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Fig. 8

rbpms2 is required for definitive female differentiation.

(A) Quantification of PGC numbers in d3 rbpms2 double mutants and non-mutant siblings. (B-C) Vasa staining to count PGCs in a representative slice from Z-series with PGCs circled in yellow in non-mutant sibling (B), and rbpms2 double mutant (C). (D-E) Transgenic germ cell marker ziwi:GFP allows visualization of comparable gonocyte development in bipotential gonads of d21 non-mutant siblings (D) and rbpms2 mutants (E). (F-G) Female (F) and male (G) gonad differentiation of control non-mutant siblings at d35. (H) rbpms2 mutant gonad with oocyte-like cells outlined in solid line, and testis-like cells outlined in dashed line. (I) rbpms2 mutant gonad resembling non-mutant male siblings.

Expression Data

Gene:	EGFP
Fish:	uc02Tg rbpms2b^{sa9329/sa9329}; rbpms2a^ae30/ae30; uc02Tg
Anatomical Terms:	germ line cell immature gonad ovary testis
Stage Range:	Days 21-29 to Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rbpms2b^{sa9329/sa9329}; rbpms2a^ae30/ae30 rbpms2b^{sa9329/sa9329}; rbpms2a^ae30/ae30; uc02Tg
Observed In:	female sex differentiation primordial germ cell
Stage Range:	Protruding-mouth to Days 30-44

Phenotype Detail

Acknowledgments

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