FIGURE

Fig. 3

ID

ZDB-FIG-181101-15

Publication

Kaufman et al., 2018 - rbpms2 functions in Balbiani body architecture and ovary fate

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

rbpms2 mutant allele stability and localization activity in somatic cells.

(A) RT-PCR to detect rbpms2a and rbpms2b maternal transcripts. Pink asterisks indicate likely nonsense mediated decay of mutant allele transcripts. (B-F) Wild-type GFP-Rbpms2a/b (B, D) and GFP-Rbpms2b^sa9329 (F) localize to granules in HEK 293 cells, while GFP-Rbpms2a^ae30 and GFP-Rbpms2b^ae32 are not localized. (G-K) Zebrafish blastula cells expressing GFP-Rbpms2 fusions. (G,H) Wild-type GFP-Rbpms2b localizes near the nucleus (H), is apparently associated with the centrosome/spindle in some cells, and (G) is in granules that are not positive for the stress granule marker Tial-1. (H) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2 (open arrowheads). (I) GFP-Rbpms2b^sa9329 localization to the centrosome/spindle but not granules. (J) GFP-Rbpms2b^ae32 and (K) GFP-Rbpms2a^ae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole.

Expression Data

Genes:	rbpms2a rbpms2b
Fish:	AB rbpms2a^ae30 (AB) rbpms2b^ae32 (AB) rbpms2b^sa9329 (AB)
Anatomical Term:	whole organism
Stage:	4-cell

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rbpms2a^ae30 (AB) rbpms2b^ae32 (AB)
Observed In:	whole organism
Stage:	4-cell

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.