FIGURE

Fig. 5

ID

ZDB-FIG-180828-32

Publication

Huo et al., 2018 - Prednisolone induces osteoporosis-like phenotypes via focal adhesion signaling pathway in zebrafish larvae

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Osteoporosis-like phenotypes caused by prednisolone through itga10 and itgbl1. (A) qRT-PCR confirmed that itga10 expression is down-regulated by prednisolone. (B) qRT-PCR confirmed that itgbl1 expression is down-regulated by prednisolone. (C) The gRNAs for itga10 and itgbl1 were designed and injected into single-cell embryos. Whole mount skeletal staining was performed on fixed tissues. (D,E) Digital image analysis of stained area, and staining density by fluorescence intensity measurement. The stained mineralized tissue was quantified using analysis software. Mean values are plotted (n=5) and the Student’s t-test was performed to determine statistical significance.

Expression Data

Genes:	itga10 itgbl1
Fish:	WT
Condition:	chemical treatment by environment: prednisolone
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + CRISPR1-itga10 WT + CRISPR1-itgbl1
Condition:	chemical treatment by environment: prednisolone
Knockdown Reagents:	CRISPR1-itga10 CRISPR1-itgbl1
Observed In:	skeletal tissue whole organism
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open