Fig. 6

Exogenous itga10 and itgbl1 capped mRNA rescue the osteoporosis-like phenotype of prednisolone. (A) After treatment with 25 µM prednisolone, whole mount skeletal staining was performed at 10 dpf. (B) itga10 capped mRNA was injected into single-cell embryos, larvae were treated with 25 μΜ prednisolone at 6 dpf and whole mount skeletal staining was conducted at 10 dpf. (C) itgbl1 capped mRNA was injected into single-cell embryos. Larvae were treated with 25 μΜ prednisolone at 6 dpf, and whole mount skeletal staining was conducted at 10 dpf. (D) itga10 and itgbl1 capped mRNAs were co-injected into single-cell embryos, larvae were treated with 25 μΜ prednisolone at 6 dpf, and whole mount skeletal staining was conducted at 10 dpf. (E) Digital image analysis of stained area and staining density by fluorescence intensity measurement, and the stained mineralized tissue was quantified using analysis software. Mean values are plotted (n=5) and the Student's t-test was performed to determine statistical significance.