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Fig. 5
Osteoporosis-like phenotypes caused by prednisolone through itga10 and itgbl1. (A) qRT-PCR confirmed that itga10 expression is down-regulated by prednisolone. (B) qRT-PCR confirmed that itgbl1 expression is down-regulated by prednisolone. (C) The gRNAs for itga10 and itgbl1 were designed and injected into single-cell embryos. Whole mount skeletal staining was performed on fixed tissues. (D,E) Digital image analysis of stained area, and staining density by fluorescence intensity measurement. The stained mineralized tissue was quantified using analysis software. Mean values are plotted (n=5) and the Student’s t-test was performed to determine statistical significance.