FIGURE

Fig. 8

ID

ZDB-FIG-180822-26

Publication

Pinto et al., 2018 - Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach

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Fig. 8

Evaluation of the specificity of dmrt2a-MO. a Alignment between wildtype dmrt2a sequence, dmrt2a^Δ100 sequence and dmrt2a-MO. The MO is represented in the sense orientation. In bold caps is represented the start codon, in green the indels and in orange the 3’end of the 5’UTR. b, c Validation of the specificity of dmrt2a-MO using (b) myod1 (14-somite stage) and (c) spaw (20-somite stage). The embryos shown illustrate the different phenotypes obtained in myod1 expression. d, d’ Evaluation of p53 levels by qPCR in (d) Region 1 (somites) and (d’) Region 2 (tailbud/LRO) after injecting dmrt2a-MO in wildtype embryos. UTR: Untranslated Region, L: left, R: right, B: bilateral, NAE: no anterior expansion, Sym: symmetric, Asym: asymmetric, ctrMO: control morpholino, ns: not significant. P-values were generated using a two-tailed t-test

Expression Data

Genes:	myod1 spaw
Fish:	TU TU + MO1-dmrt2a dmrt2a^{imm003/imm003}; dmrt2b^{imm005/imm005} dmrt2a^{imm003/imm003}; dmrt2b^{imm005/imm005} + MO1-dmrt2a
Knockdown Reagent:	MO1-dmrt2a
Anatomical Terms:	lateral mesoderm left side lateral mesoderm right side somite
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-dmrt2a dmrt2a^{imm003/imm003}; dmrt2b^{imm005/imm005} dmrt2a^{imm003/imm003}; dmrt2b^{imm005/imm005} + MO1-dmrt2a
Knockdown Reagent:	MO1-dmrt2a
Observed In:	lateral mesoderm right side left/right pattern formation somite
Stage Range:	14-19 somites to 20-25 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.