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Fig. S6

ID

ZDB-FIG-180822-27

Publication

Pinto et al., 2018 - Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach

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Fig. S6

No misexpression was found in the validated genes after dmrt2a gain and loss-of-function experiments. A1-L2? In situ hybridisation results depicting the six genes validated in this work. A1-B2? etv2 expression pattern after injecting ctrMO (A1-A1?), dmrt2a-MO (A2-A2?), and after heat-shock in control (B1-B1?) and Tg(hsp70:HA-dmrt2a) (B2-B2?). C1-D2?? foxj1b expression pattern after injecting ctrMO (C1-C1??), dmrt2a-MO (C2-C2??), and after heat-shock in control (D1-D1??) and Tg(hsp70:HA-dmrt2a) (D2-D2??). E1-F2? cyp1a expression pattern after injecting ctrMO (E1-E1?), dmrt2a-MO (E2-E2?), and after heat-shock in control (F1-F1?) and Tg(hsp70:HA-dmrt2a) (F2-F2?). G1-H2 cxcl12b expression pattern after injecting ctrMO (G1), dmrt2a-MO (G2), and after heat-shock in control (H1) and Tg(hsp70:HA-dmrt2a) (H2). I1-J2 pxdc1b expression pattern after injecting ctrMO (I1), dmrt2a-MO (I2), and after heat-shock in control (J1) and Tg(hsp70:HA-dmrt2a) (J2). K1-L2? foxc1b expression pattern after injecting ctrMO (K1, K1?), dmrt2a-MO (K2, K2?), and after heat-shock in control (L1, L1?) and Tg(hsp70:HA-dmrt2a) (L2, L2?). After dmrt2a overexpression using Tg(hsp70:HA-dmrt2a) we did not observe misexpression of the six validated genes. Upon comparison with dmrt2a-MO injected embryos, we observed changes in the expression levels of some genes (arrowheads), according to qPCR data. As depicted with asterisks, after dmrt2a-MO injection we observed only very subtle changes in the expression pattern of some genes, corresponding to the less affected genes, as quantified by qPCR. All embryos were collected between 3 and 4-somite stage (loss-of-function experiments) and between 2 h and 2 h 30 min after heat-shock (gain-of-function experiments). (A1-B2, C1-D2, E1-F2, G1-H2, I1-J2, K1-L2) Lateral view, anterior to the left. (A1?-B2?, C1?-D2?, E1?-F2?) Dorsal-anterior view, anterior to the top. (A1?-B2?, C1??-D2??, E1?-F2?, K1?-L2?) Dorsal-posterior view, anterior to the top. (C1?-D2?) Dorsal-medial view, anterior to the top. ctrMO: control morpholino. (TIF 13209 kb)

Expression Data

Expression Detail

Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.