FIGURE

Fig. S6

ID

ZDB-FIG-180817-14

Publication

Mahony et al., 2018 - Oncostatin M and Kit-Ligand Control Hematopoietic Stem Cell Fate during Zebrafish Embryogenesis

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Fig. S6

osmr morphants have reduced primitive erythropoiesis but normal primitive myelopoiesis.

(A) flk1, flt4 and dlc ISH expression at 28hpf in control or osmr morphants (B) gata1 ISH expression at 22hpf in control or osmr morphants. (C) O-dianisadine staining at 48hpf in control or osmr morphants. (D) Representative images of double transgenic flk1:eGFP/gata1:DsRed embryos injected with control or osmr morphants at 48hpf. (E) ISH analysis of macrophages (mfap expression) and neutrophils (mpx expression). All scale bars are 100μm.

Expression Data

Genes:	dlc DsRed EGFP flt4 gata1a kdrl mfap4.1 mpx
Fish:	AB AB + MO1-osmr s843Tg; sd2Tg s843Tg; sd2Tg + MO1-osmr
Knockdown Reagent:	MO1-osmr
Anatomical Terms:	dorsal aorta macrophage neutrophil nucleate erythrocyte vasculature
Stage Range:	26+ somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg; sd2Tg + MO1-osmr
Knockdown Reagent:	MO1-osmr
Observed In:	nucleate erythrocyte
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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