FIGURE

Fig. 5

ID

ZDB-FIG-180817-8

Publication

Mahony et al., 2018 - Oncostatin M and Kit-Ligand Control Hematopoietic Stem Cell Fate during Zebrafish Embryogenesis

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Fig. 5

osm and kitlgb Signal Synergistically to Expand HSCs in the CHT

(A) runx1 ISH at 28 hpf in NI embryos and kitlgb or osm mRNA-injected embryos (injected separately and together) at subliminal doses. Scale bar, 100 μm.

(B) cmyb ISH at 4 dpf in NI embryos and kitlgb or osm mRNA-injected embryos (injected separately and together) at subliminal doses. Scale bar, 100 μm.

(B′) cmyb expression analysis. Analysis is Fisher's exact test. NI versus kitlgb, p = 0.29; NI versus osm, p = 0.48; NI versus kitlgb + osm, p = 0.0001.

(D) Immunofluorescence for GFP and pH3. Arrows represent double-positive, proliferating cells. Scale, 25 μm.

(D′) Quantification of double-positive cells. (D′) Data are means ± SD and analysis is an ordinary one-way ANOVA with multiple comparisons. ANOVA p value = 0.0082. ^∗∗∗p < 0.001; ^∗∗p < 0.01; NS, p > 0.05.

Expression Data

Genes:	EGFP myb runx1
Antibody:	Ab46-h3
Fish:	AB zf169Tg
Anatomical Terms:	caudal hematopoietic tissue caudal hematopoietic tissue hematopoietic stem cell dorsal aorta
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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