FIGURE

Fig. 5

ID

ZDB-FIG-180808-9

Publication

Yu et al., 2018 - Zebrafish androgen receptor is required for spermatogenesis and maintenance of ovarian function

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Fig. 5

Proliferating germ cells were reduced, but apoptotic germ cells increased in ar-null zebrafish testes. (A, B) Immunofluorescent staining with anti-vasa antibody identified different types of spermatogenic cells in ar ^-/- testes and wildtype sibling (ar ^+/+) testes at 70 dpf or 4.5 mpf, respectively. SG, spermatogonia; SZ, spermatozoa; PSP, primary spermatocyte; SSP, secondary spermatocyte; SPD, spermatid. (C) Immunohistochemistry with anti-cyclin D1 antibody indicated that proliferating germ cells were reduced in ar ^-/- testes compared with wildtype (ar^+/+) siblings at 4.5 mpf. (D) TUNEL assay indicated increased apoptosis of germ cells in ar ^-/- testes compared with wildtype (ar ^+/+) siblings at 4.5 mpf. Mpf, months post fertilization. (E, F) Expression of ccnd2a (cyclin d2a) was down-regulated, but expression of gsdf was up-regulated in ar^-/- (ar ^{ihb1225/ihb1225}) testes compared with wildtype (ar ^+/+) sibling testes (4.5 mpf, n=3, respectively).

Expression Data

Genes:	ccnd2a ddx4 gsdf
Fish:	AB ar^{ihb1225/ihb1225} (AB)
Anatomical Terms:	spermatocyte spermatogonium testis
Stage Range:	Days 45-89 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ar^{ihb1225/ihb1225} (AB)
Observed In:	germ cell proliferation spermatocyte spermatogenesis spermatogonium testis testis germ line cell
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

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