FIGURE

Fig. S15

ID

ZDB-FIG-180611-84

Publication

Freudenblum et al., 2018 - In vivo imaging of emerging endocrine cells reveals a requirement for PI3K-regulated motility in pancreatic islet morphogenesis.

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Fig. S15

Heat shock induction of PTX expression impacts islet assembly. Related to Figure 7.

(A) hsp70:LiveActTom-PTX embryos untreated (top), or heat shocked for 30 minutes at 50% epiboly (bottom), examined at 24 hpf. Induction of PTX leads to reduced body axis length (left) and perturbed cardiac development, as evidenced by reduced cmlc2:GFP expression (center). LifeActTom, and by inference PTX, is ubiquitously induced following a heat shock (right). (B) Representative images of transgene expression in larvae quantitated in (7C), transgenic for pax6b:GFP alone (top), or pax6b:GFP in combination with hsp70:LifeActTom (middle) or hsp70:LifeActTom-PTX (bottom). Green channel alone (pax6b:GFP), posterior islets subjected to quantitative analysis are outlined (center, white line). Projection of 3D objects as identified by ImageJ plugin Particle Analyser (right).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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