FIGURE

Fig. S9

ID

ZDB-FIG-180611-78

Publication

Freudenblum et al., 2018 - In vivo imaging of emerging endocrine cells reveals a requirement for PI3K-regulated motility in pancreatic islet morphogenesis.

Other Figures

All Figure Page

Back to All Figure Page

Fig. S9

Quantitative assessment of islet assembly.

(A) Schematic illustration of the progression of islet assembly (left) and experimental design (right). (B-I) 3D-projections of pax6b:GFP;Tp1:H2BmCherry larva generated using Imaris. Dispersed mCherry⁺/GFP⁺-cells at 6 dpf (B, arrows, D-E) and clusters at 8 dpf (F, arrows, H,I). (E, I) Secondary islets in the posterior pancreas (blue surfaces), identified using Imaris. Nearby GFP⁺/mCherry^- cells (I, arrow) belong to the gut enteroendocrine system. Scale bar = 50μm. (J) Volumes of secondary islets, as analyzed using Imaris (blue surfaces in E, I), at 6 dpf (n=7) and 8 dpf (n=7) (p<0.0001, Mann-Whitney test, one-tailed). (K) GFP⁺/ H2BmCherry⁺ cells at 6 dpf and 8 dpf in samples as in (B) and (F) (p=0.25, t-test, two-tailed). The same samples were imaged at 6 dpf and 8 dpf (n=7). Results of all analyses are representative of 2 independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development