ZFIN Image: Freudenblum et al., 2018, Fig. S15

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Figure Caption

Fig. S15

Heat shock induction of PTX expression impacts islet assembly. Related to Figure 7.

(A) hsp70:LiveActTom-PTX embryos untreated (top), or heat shocked for 30 minutes at 50% epiboly (bottom), examined at 24 hpf. Induction of PTX leads to reduced body axis length (left) and perturbed cardiac development, as evidenced by reduced cmlc2:GFP expression (center). LifeActTom, and by inference PTX, is ubiquitously induced following a heat shock (right). (B) Representative images of transgene expression in larvae quantitated in (7C), transgenic for pax6b:GFP alone (top), or pax6b:GFP in combination with hsp70:LifeActTom (middle) or hsp70:LifeActTom-PTX (bottom). Green channel alone (pax6b:GFP), posterior islets subjected to quantitative analysis are outlined (center, white line). Projection of 3D objects as identified by ImageJ plugin Particle Analyser (right).

Acknowledgments

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