Fig. 6
A mechanosensory map of Tmc2b dependence in the anterior and posterior lateral lines. In this graphic of a larval zebrafish tmc2b ?/? mutant, red neuromasts have been characterized. Individual neuromasts with patterns of FM1-43FX uptake are displayed. Red hair cells take up FM1-43FX; gray hair cells do not. Below each schematized neuromast is the percentage of hair cells that load 4-Di-2-ASP (red text) in the tmc2b ?/? mutant and the percentage of fluorescence intensity of hair cells of the mutant relative to hair cells of wild-type and heterozygous animals (black). Green arrows represent the directional sensitivity preserved in the mutant based on FM1-43FX uptake. Graphs of mean microphonic potentials from neuromasts directly above each plot are displayed. For IO4, tmc2b +/??=?9.2?±?0.7??V (n?=?9), and tmc2b ?/??=?8.9?±?0.87??V (n?=?6). P value?=?0.9546. For A?P oriented posterior neuromasts, tmc2b +/+, tmc2b +/??=?8.3?±?0.37??V (n?=?20), and tmc2b ?/??=?2.1?±?0.8??V (n?=?15). ****P value?<?0.0001. For D?V oriented posterior neuromasts, tmc2b +/??=?6?±?0.49??V (n?=?4) and tmc2b ?/??=?0?±?0??V (n?=?6). ****P value?=?0.0048. P values were obtained from the Mann?Whitney test. For 4-Di-2-ASP uptake assays of SO and IO neuromasts, n???5. n values for other neuromasts are listed in Figs. 3, 4 legends |