FIGURE

Fig. 6

ID

ZDB-FIG-180502-29

Publication

Chou et al., 2017 - A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish

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Fig. 6

A mechanosensory map of Tmc2b dependence in the anterior and posterior lateral lines. In this graphic of a larval zebrafish tmc2b ^−/− mutant, red neuromasts have been characterized. Individual neuromasts with patterns of FM1-43FX uptake are displayed. Red hair cells take up FM1-43FX; gray hair cells do not. Below each schematized neuromast is the percentage of hair cells that load 4-Di-2-ASP (red text) in the tmc2b ^−/− mutant and the percentage of fluorescence intensity of hair cells of the mutant relative to hair cells of wild-type and heterozygous animals (black). Green arrows represent the directional sensitivity preserved in the mutant based on FM1-43FX uptake. Graphs of mean microphonic potentials from neuromasts directly above each plot are displayed. For IO4, tmc2b ^+/− = 9.2 ± 0.7 μV (n = 9), and tmc2b ^−/− = 8.9 ± 0.87 μV (n = 6). P value = 0.9546. For A–P oriented posterior neuromasts, tmc2b ^+/+, tmc2b ^+/− = 8.3 ± 0.37 μV (n = 20), and tmc2b ^−/− = 2.1 ± 0.8 μV (n = 15). ****P value < 0.0001. For D–V oriented posterior neuromasts, tmc2b ^+/− = 6 ± 0.49 μV (n = 4) and tmc2b ^−/− = 0 ± 0 μV (n = 6). ****P value = 0.0048. P values were obtained from the Mann–Whitney test. For 4-Di-2-ASP uptake assays of SO and IO neuromasts, n ≥ 5. n values for other neuromasts are listed in Figs. 3, 4 legends

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmc2b^cwr8/cwr8 tmc2b^cwr8/+
Observed In:	infraorbital lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound mandibular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound middle lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound opercular lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound otic lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound posterior lateral line neuromast detection of mechanical stimulus involved in sensory perception of sound
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.