FIGURE

Fig. 6

ID

ZDB-FIG-180221-31

Publication

Wakisaka et al., 2017 - An Adenosine Receptor for Olfaction in Fish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Conversion of ATP to Adenosine by Two Ecto-nucleotidases Expressed in the OE

(A) Ligand specificity (left, n = 3) and sensitivity (right, n = 4) of A2c receptor examined by cAMP production assay in CHO-K1 cells. A2c is activated only by adenosine with high specificity and sensitivity. Unpaired t test (p = 0.00000016). ∗∗∗p < 0.001.

(B) Double fluorescence in situ hybridization for A2c (magenta) and CD73 or TNAP (green) on OE sections (n = 3–4). Insets: magnified views of the boxed regions. Note the co-expression of A2c and CD73. Scale bar, 20 μm.

(C) Expression of TNAP and CD73 together with A2c in CHO-K1 cells results in A2c activation by AMP, ADP, and ATP as well as adenosine (1 mM) (n = 6). Unpaired t test (AMP, TNAP/CD73 +/–, p = 0.014; –/+, p = 0.0000044; +/+, p = 0.0000067; ADP, +/+, p = 0.0088; ATP, +/+, p = 0.0032).

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figure S5.

Expression Data

Genes:	adorb1a alpl nt5e
Fish:	WT
Condition:	chemical treatment: ATP
Anatomical Terms:	olfactory epithelium olfactory epithelium olfactory receptor cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.