ZFIN Image: Wakisaka et al., 2017, Fig. 6

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Figure Caption

Fig. 6

Conversion of ATP to Adenosine by Two Ecto-nucleotidases Expressed in the OE

(A) Ligand specificity (left, n = 3) and sensitivity (right, n = 4) of A2c receptor examined by cAMP production assay in CHO-K1 cells. A2c is activated only by adenosine with high specificity and sensitivity. Unpaired t test (p = 0.00000016). ∗∗∗p < 0.001.

(B) Double fluorescence in situ hybridization for A2c (magenta) and CD73 or TNAP (green) on OE sections (n = 3–4). Insets: magnified views of the boxed regions. Note the co-expression of A2c and CD73. Scale bar, 20 μm.

(C) Expression of TNAP and CD73 together with A2c in CHO-K1 cells results in A2c activation by AMP, ADP, and ATP as well as adenosine (1 mM) (n = 6). Unpaired t test (AMP, TNAP/CD73 +/–, p = 0.014; –/+, p = 0.0000044; +/+, p = 0.0000067; ADP, +/+, p = 0.0088; ATP, +/+, p = 0.0032).

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Figure S5.

Figure Data

Expression / Labeling details

Acknowledgments

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