FIGURE

Fig. 6

ID

ZDB-FIG-180118-11

Publication

Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development

Other Figures

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Fig. 6

Imaging MHB development in real time. To visualize MHB development in real time, embryos from the otx2:tRFP reporter line were mounted and imaged dorsally from 17 to 22 hpf. Tissue sections covering about 40 μm were chosen with a z interval of 2 μm. Images were acquired at 6 min intervals. The otx2:tRFP + cells in the midbrain are clearly visible and cell movements can be followed during ventricular space opening and folding of the neural tube at the MHB. In addition, cellular rearrangements in delaminating neural crest cells and retinal cells are also visible. Time in minutes: seconds. Scale bar: 50 μm.

Expression Data

Gene:	RFP
Fish:	otx2b^tud41Tg (AB)
Anatomical Terms:	midbrain neural crest midbrain optic cup cell
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.