FIGURE

Fig. 5

ID

ZDB-FIG-180118-10

Publication

Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Reporter knock-in into the otx2 locus does not affect endogenous otx2 expression. otx2:venus and otx2:tRFP fish were crossed and sorted into either single (Venus⁺ or tRFP⁺) or double positive (Venus⁺ and tRFP⁺) embryos at 24 hpf. Images were taken from live embryos anesthetized in MS-222. Panel (A) fluorescent images of the single- and double-positive embryos show no morphological abnormalities. Images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Scale bar: 50 μm. Images were taken from live embryos anesthetized in MS-222. Panel (B) Double in situ hybridization for otx2 (blue) and the MHB marker pax2a (red) show no differences in gene expression pattern or morphology of the MHB region between the single- and double-positive groups. Mb, midbrain; Hb, hindbrain. Scale bar 50 μm.

Expression Data

Genes:	otx2b pax2a RFP Venus
Fish:	otx2b^tud40Tg/+ (AB) otx2b^tud41Tg/+ (AB) otx2b^tud40Tg/+; otx2b^tud41Tg/+ (AB)
Anatomical Terms:	midbrain midbrain hindbrain boundary
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.