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Fig. 6
Imaging MHB development in real time. To visualize MHB development in real time, embryos from the otx2:tRFP reporter line were mounted and imaged dorsally from 17 to 22 hpf. Tissue sections covering about 40 μm were chosen with a z interval of 2 μm. Images were acquired at 6 min intervals. The otx2:tRFP + cells in the midbrain are clearly visible and cell movements can be followed during ventricular space opening and folding of the neural tube at the MHB. In addition, cellular rearrangements in delaminating neural crest cells and retinal cells are also visible. Time in minutes: seconds. Scale bar: 50 μm.
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Full text @ Front. Neuroanat.