FIGURE

Fig. 2

ID

ZDB-FIG-180118-8

Publication

Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development

Other Figures

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Fig. 2

Targeted knock-in of Venus fluorescent protein into the otx2 locus. Expression of Venus fluorescent protein at 24 hpf. Images were taken from live embryos anesthetized in MS-222. (A) Left panel shows dorsal view with the anterior of the embryo facing upwards, right panel is a merged image of the fluorescent and transmitted light channels. Venus is expressed in the retina and midbrain, sharply abutting the MHB (dotted line). (B) Lateral view of an embryo expressing Venus. Left panel shows fluorescent channel and right panel shows a merged image of the fluorescent and transmitted light channels. All images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Mb, midbrain; Hb, hindbrain. Scale bar: 100 μm.

Expression Data

Gene:	Venus
Fish:	otx2b^tud40Tg (AB)
Anatomical Terms:	midbrain midbrain hindbrain boundary optic cup
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.