FIGURE

Fig. 5

ID

ZDB-FIG-171018-14

Publication

Leerberg et al., 2017 - Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Fgf24 regulates gene expression of somatic gonad cells.

(A-J) Single plane confocal micrographs of whole-mount larval gonads after fluorescent in situ hybridization detecting SGC markers (red). Nuclei labeled with DAPI (blue), germ cells labeled with Vasa (green). gata4 (A), nr5a1a (C), wt1a (E), cyp19a1a (G), and amh (I) are expressed in somatic gonad cells (SGCs) of wild-type larval gonads, whereas only wt1a can be detected in SGCs of fgf24 mutants (F). (K-M) Double fluorescent in situ hybridization and Vasa antibody staining (blue) of 12 dpf wild-type gonads (outlined by white dotted line). etv4, cyp19a1a, and amh are expressed in non-overlapping SGC domains (n = 12, 12, and 3 for K, L, and M, respectively). In E, arrows denote inner SGCs; arrowheads denote outer SGCs. A-M are sagittal optical sections with anterior to the left. Scale bars = 20 μm.

Expression Data

Genes:	amh cyp19a1a ddx4 etv4 gata4 nr5a1a wt1a
Antibody:	Ab1-ddx4
Fish:	AB fgf24^uc47/uc47 (AB)
Anatomical Terms:	germ line cell gonad primordium somatic cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf24^uc47/uc47 (AB)
Observed In:	gonad primordium somatic cell
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.