Fig. 4
- ID
- ZDB-FIG-171006-34
- Publication
- Du et al., 2014 - Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1
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Knockdown of the dvr1 gene leads to abnormal C&E movements. (A–H) Whole-mount in situ hybridization for fgf8a, dlx3b, ctsl1b, myod1 and ntla were performed in control and dvr1 moATG morphants at the indicated developmental stages. (I–P) Whole-mount in situ hybridization for ntla, dlx3b, ctsl1b, egr2b and myod1 were performed in control and dvr1 moSB morphant embryos at the indicated developmental stages. The percentage of phenotypes were as follows: (J) 17/19 (ntla), (L) 42/44 (dlx3b), 13/44 (ctsl1b), (N) 23/25 (egr2b), (P) 34/34 (myod1). Red double-headed arrows denote the width of the presumptive hindbrain in the lateral view or the width of rhombomeres 3 and 5 in the dorsal view. Green dotted lines indicate the angle between the two sides of the neural plate. Black and blue double-headed arrows show the width and length of somites in E-F and O-P, respectively. Black double-headed arrows in I and J indicate the length of the notochord. Embryos were dorsal view in left side of A-B, E-F, I-J and M-P with head to the top, animal view in C-D and K-L with head to the left, lateral view in right side of A and B with head to the top, lateral view in left side of G and H and dorsal view in right side of G and H with head to the left. (Q) The C&E defects were specifically caused by Dvr1 inhibition. Whole-mount mRNA in situ hybridization of the expression pattern of myod1 in control, dvr1 moATG-injected and dvr1 moATG plus dvr1 mRNA-injected embryos at 9-somite stage. |
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Stage Range: | Bud to 20-25 somites |
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Stage Range: | Bud to 20-25 somites |
Reprinted from Developmental Biology, 392(2), Du, T.T., Xu, P.F., Dong, Z.W., Fan, H.B., Jin, Y., Dong, M., Chen, Y., Pan, W.J., Ren, R.B., Liu, T.X., Deng, M., Huang, Q.H., Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1, 233-44, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.