Fig. 3

Forced expression of dvr1 impairs epiboly and C&E movements. (A–F) Embryos were either uninjected or injected with 80, 40, 20, 15 or 10 pg dvr1 mRNA at the one-cell stage and were imaged at 10 hpf. Base on the severity and stage of morphological phenotypes, the embryos were grouped into normal bud stage (blue), 50% epiboly (red), 70% epiboly (orange), 90% epiboly (green) and C&E defects (gray). Statistical analyses of the phenotypes are shown in F. The dotted lines in A and E indicate the angle between the most anterior and posterior ends of the body axis. The arrows in B-D indicate the front edge of epiboly process. All embryos were lateral view. (G) The average extension angle of uninjected embryos and embryos injected with 10 pg dvr1 mRNA which had impaired extension (approximately 40% of total 10 pg dvr1 mRNA injected embryos) was plotted. The statistical significance of this difference is indicated by asterisks (****, P<0.0001). (H-M) Embryos at one-cell stage were injected with control or dvr1 mRNA (50 pg EGFP mRNA or 10 pg dvr1 mRNA) and stained with RNA probes for ntla, ctsl1b, dlx3b and myod1 at 10 hpf. White and black double-headed arrows denote the width and length of the notochord and somites, respectively. The green dotted lines indicate the angle between the two sides of the neural plate. Embryos were dorsal view in H-I and L-M with head to the top and animal view in J and K with head to the left.