Fig. 1

Setdb2 knockdown induces defects in C&E movements during gastrulation. (A–G) Whole-mount in situ hybridization and statistical analyses of the expression patterns of ntla, ctsl1b, dlx3b and egr2b at the indicated developmental stages in embryos injected with setdb2 control mo or setdb2 mo. Insets show magnified views of the relative position of ctsl1b and dlx3b. The dorsal views on embryos in A and B were flat-mounted. Embryos were animal views in C and D with head to the left and dorsal views in E and F with head to the top. L1 and L2, the length and width of the notochord at 10 hpf; L3, the length of the posterior shift of prechordal plate relative to the overlying neural plate; Angle, between the two sides of neural plate; L4, the width of rhombomeres 3 and 5. Asterisks indicate statistically significant differences (*, P<0.05; **, P<0.01; ***, P<0.001). (H–K) Representative confocal images of notochord cells in setdb2 control embryos (n=4 embryos, 80 cells) and setdb2 morphants (n=4 embryos, 80 cells) co-injected at the one-cell stage with 100 pg mRNA coding for membrane-targeted RFP. Anterior is upwards. The boundaries of the notochord are marked with dotted white lines. Length-width ratio (LWR) of notochord cells and mediolateral alignment (ML alignment) at the 3-somite stage were measured with ImageJ2X. The rose diagrams were drawn using Rose.NET. Scale bar: 20 μm. The statistical significance of difference is indicated by asterisks (****, P<0.0001).