FIGURE

Fig. S8

ID

ZDB-FIG-170912-31

Publication

Lamont et al., 2016 - The LIM-homeodomain transcription factor Islet2a promotes angioblast migration

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Fig. S8

Loss of isl2 compensates for increased ISV angioblast number caused by loss of notch signaling by DAPT (A-C) isl2 expression is upregulated after treatment with DAPT (B) as compared to untreated control embryos (A). (C) Relative expression levels of isl2 and flt4 were quantitated by qPCR. Expression of isl2 and flt4 was increased by 2.2-fold in DAPT-treated embryos. (D-H) Representative embryos showing the number of nuclei per ISV at 30 hpf. (D) Wild type control embryos treated with DMSO, (E) isl2^ATG MO, (F) DAPT treatment, (G), DAPT and isl2ATG MO Lo, and (H) DAPT with isl2^ATG MO Hi. (I) Quantitation of the average number of cells in single ISVs in DMSO treated control embryos (4.3 ± 1.1), isl2^ATG morphants (1.8 ± 0.7), DAPT treated embryos (5.0 ± 1.4), DAPT treatment with isl2^ATG Lo (3.7 ± 1.0), or DAPT treatment with isl2^ATG MO Hi (3.6 ± 1.4) (* refers to p< 0.05, ** refers to p<0.005, and *** refers to p<0.0001 by an unpaired student's t-test. Scale bars represent 100 µm in A, B and 50 µm in D-H.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 414, Lamont, R.E., Wu, C.Y., Ryu, J.R., Vu, W., Davari, P., Sobering, R.E., Kennedy, R.M., Munsie, N.M., Childs, S.J., The LIM-homeodomain transcription factor Islet2a promotes angioblast migration, 181-92, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.