Fig. 1

Absence of obvious morphological or vascular defects in hif-1aa and hif-1ab single mutants.

(a,b) Schematic representation of the following proteins: WT and mutant (bns89 allele) Hif-1aa, WT and mutant (bns90 allele) Hif-1ab. bHLH, basic helix-loop-helix domain; PAS-A/B, PAS domains; ODD, oxygen degradation dependent domain; N-TAD, N-terminal transactivation domain; C-TAD, C-terminal transactivation domain. Red box before the stop codon represents missense sequence. (c) qPCR analysis of hif-1aa expression in WT siblings and hif-1aa^bns89 mutants at 48 hpf. (d) qPCR analysis of hif-1ab expression in WT siblings and hif-1ab^bns90 mutants at 48 hpf. Values represent mean±s.d., n=3 biological replicates, (*P<0.05; **P<0.01; t-test). (e) Representative brightfield images and maximal intensity projections of confocal z-stacks of Tg(kdrl:EGFP);Tg(gata1:DsRed) WT siblings and hif-1aa and hif-1ab mutants at 72 hpf; lateral views. n=3 clutches. CA, caudal artery; CV, caudal vein; DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; PCV, posterior cardinal vein. Scale bar, 200 μm.