FIGURE

Fig. 7

ID

ZDB-FIG-170907-36

Publication

Gerri et al., 2017 - Hif-1α regulates macrophage-endothelial interactions during blood vessel development in zebrafish

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Fig. 7

Macrophages do not appear to assist in vessel repair in hif-1α mutants.

(a) Schematic representation of the experiment shown in b–d. (b) Maximal intensity projections of time-lapse confocal images of a Tg(kdrl:EGFP);Tg(mpeg:mCherry) WT sibling treated with DMOG starting at 48 hpf. Yellow arrowheads point to a vessel rupture and white arrowheads to macrophages. (c) Maximal intensity projections of time-lapse confocal images of a Tg(kdrl:EGFP);Tg(mpeg:mCherry) hif-1α mutant treated with DMOG starting at 48 hpf. Yellow arrowheads point to a vessel rupture and subsequent regression. (d) Quantification of blood vessel ruptures repaired in the presence (w/) or absence (w/o) of macrophages as well as those not repaired in WT and hif-1α^−/− embryos at 65 hpf. All images represent lateral views. Bars represent mean±s.e.m., n=6 embryos from three different clutches, (*P<0.05; t-test). Scale bar, 50 μm.

Expression Data

Genes:	EGFP mCherry
Fish:	s843Tg; ump2Tg hif1ab^bns90/bns90; hif1aa^bns89/bns89; s843Tg; ump2Tg
Condition:	chemical treatment by environment: dimethyloxalylglycine
Anatomical Terms:	intersegmental vessel macrophage trunk vasculature
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hif1ab^bns90/bns90; hif1aa^bns89/bns89; s843Tg; ump2Tg
Condition:	chemical treatment by environment: dimethyloxalylglycine
Observed In:	intersegmental vessel regeneration
Stage Range:	Long-pec to Pec-fin

Phenotype Detail

Acknowledgments

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