Fig. 4
- ID
- ZDB-FIG-170609-39
- Publication
- Theodore et al., 2017 - Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization
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Mmp2 Facilitates HSPC Budding and Migration via Extracellular Matrix Digestion (A) Confocal images show fibronectin-rich ECM in the VDA (31 hpf): α-fibronectin antibody, green; DAPI (nuclei) staining, blue. Arrows point to cells between the dorsal aorta (DA) and posterior cardinal vein (PCV). (B) High-magnification imaging of Tg(itga2b:gfp) embryos at 48 hpf shows Cd41:GFP+ HSPCs embedded in fibronectin-rich ECM between the DA and PCV. (C) ARP (10 μM) treatment (12–36 hpf) increased fibronectin staining in the AGM (right) compared with controls at 36 hpf (left) (n ≥ 10 embryos/condition). Arrows mark areas of high fibronectin staining. (D) ARP exposure (12–72 hpf) caused sustained cmyb AGM expression in WT siblings, not in fibronectin (fn1−/−) mutants at 72 hpf. Brackets mark anterior/posterior extent of AGM region. (E) Qualitative phenotypic distribution of embryos from (D) scored for presence or absence of cmyb in the AGM at 72 hpf (n ≥ 20/condition). WT, wild-type. Scale bars, 20 μm (A), 10 μm (B), 30 μm (C), and 100 μm (D). |
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| Stage Range: | Prim-15 to Protruding-mouth |
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| Stage Range: | Prim-25 to Protruding-mouth |