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Fig. 4
Mmp2 Facilitates HSPC Budding and Migration via Extracellular Matrix Digestion
(A) Confocal images show fibronectin-rich ECM in the VDA (31 hpf): ?-fibronectin antibody, green; DAPI (nuclei) staining, blue. Arrows point to cells between the dorsal aorta (DA) and posterior cardinal vein (PCV).
(B) High-magnification imaging of Tg(itga2b:gfp) embryos at 48 hpf shows Cd41:GFP+ HSPCs embedded in fibronectin-rich ECM between the DA and PCV.
(C) ARP (10 ?M) treatment (12?36 hpf) increased fibronectin staining in the AGM (right) compared with controls at 36 hpf (left) (n ? 10 embryos/condition). Arrows mark areas of high fibronectin staining.
(D) ARP exposure (12?72 hpf) caused sustained cmyb AGM expression in WT siblings, not in fibronectin (fn1?/?) mutants at 72 hpf. Brackets mark anterior/posterior extent of AGM region.
(E) Qualitative phenotypic distribution of embryos from (D) scored for presence or absence of cmyb in the AGM at 72 hpf (n ? 20/condition).
WT, wild-type. Scale bars, 20 ?m (A), 10 ?m (B), 30 ?m (C), and 100 ?m (D).