FIGURE

Fig. 1

ID

ZDB-FIG-170609-36

Publication

Theodore et al., 2017 - Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization

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Fig. 1

Inflammatory Activity Regulates Mmp2 and Mmp9 Expression in the VDA

(A) runx1, mmp2, and mmp9 expression was enhanced following exposure (36–42 hpf) to CAY10397 (10 μM) but decreased by indomethacin (10 μM), as determined by qPCR (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; 40 pooled embryos/condition × 3 replicates).

(B) CAY10397 (12–36 hpf) increased mmp2 and mmp9 expression, while indomethacin (Indo) diminished expression by WISH (n ≥ 20/condition).

Arrowheads mark mmp9⁺ cells; brackets mark dorsal/ventral boundaries of the VDA and CV. Scale bars, 100 μm.

Expression Data

Genes:	mmp2 mmp9 runx1
Fish:	WT
Conditions:	chemical treatment by environment: CAY10397 chemical treatment by environment: indometacin
Anatomical Terms:	trunk trunk mesenchyme trunk vasculature trunk vasculature cell whole organism
Stage Range:	14-19 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment by environment: CAY10397 chemical treatment by environment: indometacin
Observed In:	trunk whole organism
Stage Range:	Prim-25 to High-pec

Phenotype Detail

Acknowledgments

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