FIGURE

Fig. 7

ID

ZDB-FIG-170424-10

Publication

Schmitner et al., 2017 - ptf1a⁺ , ela3l^- cells are developmentally maintained progenitors for exocrine regeneration following extreme loss of acinar cells in zebrafish larvae.

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Fig. 7

Proliferation of exocrine cells during regeneration is dependent on Wnt signaling. Confocal projections of Tg(ela:casp8) crossed to Tg(hsp70l:dkk1-eGFP) and treated with 5 µM Dim. During regeneration (4-6 dpa or 6-8 dpa) larvae were heat shocked twice to induce dkk1 and they were additionally injected with EdU. (A) Timeline indicating treatments and heat shock (flames). (B) Control littermate negative for dkk1-eGFP. (C) Larvae expressing dkk1-eGFP showed reduced numbers of EdU⁺ cells. Volume of E2Crimson cells (D) and EdU⁺ E2Crimson⁺ cells (E) in larvae expressing dkk1-eGFP and controls at 6 and 8 dpa during regeneration, showing that the induced expression of dkk1 reduces the number of EdU⁺ exocrine cells (N>4 larva for each time point). Mean+ s.e.m.; **P<0.001. (F) Model of exocrine cell regeneration after complete removal of exocrine cells. Scale bar: 20 µm.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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